Cat. No.
MABL-736
Application
in vitro, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
CB-16
From
Recombinant Antibody
Specificity
This antibody is specific for the CD16 protein.
Alternative Names
FCGR3A; CD16-II; CD16a; FcR-10; CD16a antigen; IgG Fc receptor III-2; Fc-gamma RIII-alpha; Low affinity immunoglobulin gamma Fc region receptor III-A
UniProt
P08637
Immunogen
The original antibody was raised by immunizing a BALB/c mouse with CD16.
Application Notes
The original version of this antibody was used for flow cytometry on human neutrophils (Bankey et al., 2011; PMID:20123111) on human bone marrow cells (Allan et al., 2010; PMID:20540115), on PBMCs (Ingersoll et al., 2010; PMID:19965649), on differentiated pluripotent stem cells (Rönn et al., 2015; PMID:25680478), and on whole blood leukocytes (Veras et al., 2020; PMID:32926098).This antibody was used for a whole blood intracellular cytokine assay on NK cells, it showed a very strong response to the antibody (Violari et al., 2019; PMID：30679439). This antibody was used to stimulate NK cells, and its ability to trigger activation and expansion of peripheral blood NK cells (Kim et al., 2023; PMID:37302991).
Antibody First Published
Ingersoll et al. Comparison of gene expression profiles between human and mouse monocyte subsets Blood. 2010 Jan 21;115(3):e10-9. PMID:19965649
Note on publication
The original paper studied the similarity between the two main monocyte subsets in human and mouse blood.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
