Cat. No.
MABL-737
Application
IP, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
DJ130c
From
Recombinant Antibody
Specificity
This antibody recognises an epitope in the first membrane-distal domain of both CD16a and CD16b. CD16 is a low affinity receptor for IgG, and plays an important role in mediating ADCC by NK cells.
Alternative Names
CD16; Low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; CD16; CD16a; CD16b; FCGR3; FCGR3A; FCGR3B; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc- gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; Fc-gamma RIII-beta; Fc-gamma RIIIb; FcRIIIb
UniProt
P08637; O75015
Immunogen
This antibody was raised by immunising mice with CD16 antigen.
Application Notes
DJ130c may be used in identification and characterisation of various cancers. For instance, one group utilised this antibody in the analysis of expression profiles of gastric cancer xenografts exposed to cisplatin and considerable alteration in the expression level of CD16 was observed (Cai et al., 2017). Another group used this antibody to identify and compare different subpopulations of macrophages and their potential relation to acute heart transplant rejection, both via immunofluorescence and immunohistochemistry in tissue samples (van den Bosch et al., 2017).
Antibody First Published
Schmidt et al. CD16 cluster workshop report. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 805-806
Note on publication
This report describes the characterisation of DJ130c antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
