Cat. No.
MABL-734
Application
in vitro, in vivo, SPR, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MEDI3622
From
Recombinant Antibody
Specificity
This atibody is specific for a unique surface loop of sIVa-sIVb β-hairpin on ADAM17.
Alternative Names
CSVP; TACE; ADAM17; ADAM 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; Disintegrin and metalloproteinase domain-containing protein 17
UniProt
P78536
Immunogen
This antibody was raised by panning the CAT library against ADAM17.
Application Notes
The original antibody was used for flow cytometry on different variants of ADAM17 (Peng et al., 2016; PMID：27610476), and on T4-2 cells (Rios-Doria et al., 2015; PMID：25948294). This antibody was used for surface plasmon resonance on ADAM17. This antibody was used for biolayer inferometry on ADAM17 (Peng et al., 2016; PMID：27610476). This antibody was used in vivo on mice with implanted tumors, it showed the ability to strongly decrease the tumor growth and volume. The influence of this antibody on TNF alpha shedding was assessed, it inhibited the shedding. A similar experiment was also perform for EGFR ligand shedding and yielded similar results. This antibody was used for ELISA on ADAM17 (Rios-Doria et al., 2015; PMID：25948294). This antibody was added to NK cells and NK92 cells stimulated with different tumor cell lines, it showed that the antibody blocks ADAM17 activity in NK cells but not the activation of ADAM17. The antibody was also shown to enhance IFN gamma production in NK cells (Mishra et al., 2018; PMID：29978334). This antibody was used in vitro in tumor spheroids generated from chicken embryos, the antibody was used in combination with irradiation to treat the tumor. This antibody was used in vivo on murine tumor models established by injecting A549 cells in mice, it was used in tandem with irradiation. The antibody showed inhibition of the tumor growth (Tschanz et al., 2021; PMID：36860547).
Antibody First Published
Rios-Doria et al. A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways Mol Cancer Ther. 2015 Jul;14(7)：1637-49.PMID:25948294
Note on publication
The original paper reports the discovery of a potent and specific ADAM17 inhibitory antibody, MEDI3622, which induces tumor regression or stasis in many EGFR-dependent tumor models.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
