Cat. No.
MABL-716
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Pig, Human, Rhesus Monkey, Cynomolgus Monkey
Clone No.
UCH-M1
From
Recombinant Antibody
Specificity
This antibody is specific for human CD14, which is expressed strongly by macrophages and most tissue monocytes. It cross-reacts with non-human primate and pig CD14.
Alternative Names
UCHM1; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
UniProt
P08571
Immunogen
Human thymocytes followed by peripheral blood mononuclear cells.
Application Notes
This antibody has been used to specifically stain monocytes and macrophages in cryostat sections of large cell lymphoma using an indirect immunoperoxidase technique (Addis et al, 1986) and cryostat lymph node sections (Salisbury et al, 1985). It has also been used to stain acetone-fixed cytocentrifuge preparations of a cell line derived from a patient with Hodgkin's disease (Jones et al, 1985). This antibody has been employed in an immunofluorescence analysis to assess CD14 expression in porcine epithelial cells (Liu et al, 2001), and in indirect immunofluorescence staining of cryostat thymus sections (Marinova et al, 2008). This antibody has also been used in Western blotting, and to deplete soluble CD14 from human sera through an immunoadsorption method (Shimizu et al, 2004).
Antibody First Published
Jones et al. Phenotypic analysis of an established cell line derived from a patient with Hodgkin's disease (HD) Hematol Oncol. 1985 Apr-Jun;3(2):133-45. PMID:4018743
Note on publication
Describes the use of this antibody in IHC analysis
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
