Cat. No.
MABL-715
Application
WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Pig, Human
Clone No.
MiL2
From
Recombinant Antibody
Specificity
This antibody is specific for porcine CD14.
Alternative Names
Monocyte antigen; Monocyte differentiation antigen CD14; Myeloid cell- specific leucine-rich glycoprotein
UniProt
P08571
Immunogen
The original antibody was raised by immunizing a mouse with porcine CD14.
Application Notes
Western blot was performed using this antibody on proteins (US20170107294). This antibody was used for flow cytometery on fresh human or porcine whole blood (US20170107294) (Thorgersen et al, 2009; pmid:19047409). The biological effect of this antibody on the inflammatory response of piglets was tested (US20170107294). ELISA was performed on C1q and human FcγRs using this antibody (US20170107294). Immunofluorescence was performed on both PBMC and whole blood samples using this antibody (Zelnickova et al, 2007; pmid:17720184). Immunofluorescent staining was performed on cardiac and diaphragmatic lung lobes using this antibody (Gucht et al, 2005; pmid:15802956).
Antibody First Published
Gucht et al. Porcine reproductive and respiratory syndrome virus infection increases CD14 expression and lipopolysaccharide-binding protein in the lungs of pigs. Viral Immunol.2005;18(1):116-26. PMID:15802956
Note on publication
The levels of CD14 expression and LPS-binding protein (LBP) in the lungs of pigs throughout a PRRSV infection were quantified.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
