Cat. No.
MABL-717
Application
ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
10H10 (TF9-10H10)
From
Recombinant Antibody
Specificity
This antibody binds the human tissue factor heavy chain protein (huTFh) and specifically binds huTFh polypeptide p1-30.
Alternative Names
TF; F3; Tissue factor; Coagulation factor III; Thromboplastin
UniProt
P13726
Immunogen
The original antibody was generated by subcutaneously immunizing a mouse with denatured human TF protein in R-700 adjuvant.
Application Notes
Tissue factor (TF), one of the cell-surface initiators of blood coagulation and has been identified as a critical controlling molecule in hemostasis, thrombosis and inflammation. This antibody is recommended for detection of tissue factor in a blood sample. This is a non neutralizing antibody, which is capable of binding human tissue factor heavy chain protein but does not neutralize the ability of tissue factor to initiate coagulation. This antibody was used to study the interaction between factor VII and tissue factor in solution using ELISA (PMID: 1874730). This antibody is also reported to be used to target TF pathway as a possible treatment strategy for glioblastoma therapy (PMID: 23384223). This antibody was used to study expression of TF in human glioma using immunofluorescence staining for identification of TF in U251 glioma cell line, normal brain specimens, and glioma surgical specimens (PMID: 12135696). This antibody was also used in the detection of TF in normal and abnormal human mammary glands using immunohistochemistry (PMID: 1514249).
Antibody First Published
Morrissey et al. Monoclonal antibody analysis of purified and cell-associated tissue factor. Thromb Res. (1988); 52(3):247-61. PMID:3194899
Note on publication
Describes the study on interaction between factor VII and TF using a library of murine antibodies that have specificity for TF.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
