Cat. No.
MABL-699
Application
in vitro, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
WM15
From
Recombinant Antibody
Specificity
This antibody is specific for human CD13, but its epitope is destroyed during the process of formalin fixation and paraffin embedding.
Alternative Names
gp150; AP-M; AP-N; hAPN; ANPEP; APN; PEPN; Alanyl aminopeptidase; Aminopeptidase N; Aminopeptidase M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13
UniProt
P15144
Immunogen
The orignal antibody was raised by immunizing a mouse with human CD20.
Application Notes
The original antibod was used for immunohistochemsitry on a range of human inflammatory tissue specimens. This antibody was used for flow cytometry on CD31+ CD45- endothelial cells identified with mouse anti-human FITC-CD31 (Di Matteo et al., 2011; PMID:21339174), and on he human monocytoid cell line U937 (Löhn et al., 2002; PMID:11999577). This antibody was used for an in vitro wound scratch assay on human fibroblasts, it showed a 42% decrease in cell migratory activity. This antibody was used for a proliferation assay on human fibroblasts, it showed that the antibody did not affect the proliferation of fibroblasts (Lai et al., 2010; PMID:20589526).
Antibody First Published
Löhn et al. Cell cycle retardation in monocytoid cells induced by aminopeptidase N (CD13) Leuk Lymphoma. 2002 Feb;43(2):407-13. PMID:11999577
Note on publication
In the original paper the physiologiucal role of CD13 expression on human leucocytes was investigated.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
