Cat. No.
MABL-700
Application
Blocking, crystallisation, Functional Studies, IP, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BION-1
From
Recombinant Antibody
Specificity
Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL- 5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.
Alternative Names
CSF2RB; CD131; IL3RB; IL-3R common beta subunit; IL5RB; colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage); SMDP5; Common Beta-Chain; CDw131; cytokine receptor common subunit beta; beta-c; CD131; Beta Common Chain
UniProt
P32927
Immunogen
BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells.Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method.MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
Application Notes
BION-1 has been shown the block eosinophil production, survival, and activation stimulated by IL-5 as well as by GM-CSF and IL-3, thereby influencing inflammation (Sun et al., 1999; PMID: 10477723). Further, BION-1 has been used in immunohistochemistry experiments in order to examine the presence of GM-CSF-receptor protein in human 8-cell embryos and blastocysts (Sjoblom et al., 2002; PMID: 12444058). In addition, a fab fragment derived from BION-1 has been used in the crystallisation of the common beta-chain to analyse the structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor (Rossjohn et al., 2000; PMID: 10753826).
Antibody First Published
Sun et al. Simultaneous Antagonism of Interleukin-5, Granulocyte- Macrophage Colony-Stimulating Factor, and Interleukin-3 Stimulation of Human Eosinophils by Targetting the Common Cytokine Binding Site of Their Receptors Blood, Vol 94, No 6 (September 15), 1999: pp 1943 -1951 PMID:10477723
Note on publication
Describes the presence of the common beta-chain in various cell surface receptors and its role in receptor activation for inflammation. The use of BION-1 as an antagonist of IL-5, GM-CSF and IL-3 signalling through binding the common beta-chain is also outlined in the context of blocking eosinophil activation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
