Cat. No.
MABL-698
Application
IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
22A5
From
Recombinant Antibody
Specificity
This antibody is specific for CD13, with the same reactivity as MY7 and 15D6 antibodies
Alternative Names
Alanyl Aminopeptidase; Microsomal Aminopeptidase; Aminopeptidase M; Myeloid Plasma Membrane Glycoprotein CD13; GP150; CD13; PEPN; ANPEP; APN;
UniProt
P15144
Immunogen
Female BALB/c mice were primed by intraperitoneal immunization with OC-containing cell suspensions from OCL tumors. Supernatants from growth-positive cultures were screened initially for antibody on acetone-fixed OCL tumor imprints using the indirect immunofluorescent techniques.
Application Notes
This antibody has been used for immunofluorescent staining of CD13 in human T cell lymphoma lines, observed by confocal fluorescence microscopy (Murray 1995). 22A5 may be useful for the diagnosis and immunophenotyping of leukaemia.
Antibody First Published
Michael A. Morton, Denise Lewis, Katrina McNulty, Jean A. S. Pringle, and Tim J. Chambers Monoclonal Antibodies to Osteoclastomas (Giant Cell Bone Tumors) :Definition of Osteoclast-specific Cellular Antigens CANCER RESEARCH 45, 5663-5669, November 1985] PMID:4053038
Note on publication
Addresses the cellular origin of OCs, arising from a hemopoietic stem cell rather than from conventional blood cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
