Cat. No.
MABL-697
Application
antagonist, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
GR34
From
Recombinant Antibody
Specificity
This antibody binds human IL-7R.
Alternative Names
IL7R; IL7Rα; IL-7Rα; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R-alpha; IL-7RA; CDw127
UniProt
P16871
Immunogen
The original parental clone was generated by immunizing BALB/c mice with Pre-Alp, a t(l;19) pre-B leukemic cell line.
Application Notes
This IL-7R antagonist antibody is a recombinant engineered version of clone R34.34. The binding affinity of this antibody for human IL-7R was checked using ELISA. Antibody GR34 (mouse IgG1 version) was determined to inhibit pSTAT5 signalling with an IC50of 0.22μg/ml. The mouse IgG1 version had an affinity for the IL-7Rα EDC of 15.3nM (KD) and a Kd of 8.75X10-4. The mouse IgG1 version was capable of binding to IL-7Rα expressed on CHO cells with an EC50 of 0.27μg/ml, and blocked IL-7/IL-7Rα with an IC50of 2.29μg/ml (WO2010017468).
Antibody First Published
Leung et al. Treatment of autoimmune and inflammatory disease. WO2010017468
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
