Cat. No.
MABL-625
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
canine, Caprine, Feline, Hamster, Monkey, Rabbit, Rat, Xenopus, Zebrafish, Human, Mouse
Clone No.
R2G
From
Recombinant Antibody
Specificity
The original antibody is specific for cardiotin. Cardiotin is a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle.
Alternative Names
UniProt
Immunogen
The original antibody was generated by immunizing a mouse with a total protein extract of chicken gizzard.
Application Notes
The antibody could detect cardiotin on microsomal swine heart samples by western blot analysis (Schaart et al., 1997; PMID: 9042960). The antibody revealed cardiotin in the longitudinal sarcoplasmic reticulum in normal atrial myocardium of goats in sinus rhythm by immunofluorescence staining (Ausma et al., 1997; PMID: 9327732). The antibody recognized cardiotin in cardiomyocytes by immunocytochemistry (Dispersyn et al., 2001; PMID: 11470462). The antibody was employed in immunohistochemistry for the detection of cardiotin (Dispersyn et al., 2002; PMID: 12042006 and Ausma et al. 2001; PMID: 11735256).
Antibody First Published
Schaart et al. Characterization of cardiotin, a structural component in the myocard Eur J Cell Biol. 1993 Oct;62(1):34-48. PMID:8269977
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
