Cat. No.
MABL-524
Application
CHIP, IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Species-independent
Clone No.
BK-1/39
From
Recombinant Antibody
Specificity
This antibody recognises biotin (vitamin B7). It is a water-soluble vitamin which serves as a cofactor of important metabolic enzymes involved in processing of carbohydrates, lipids and amino acids as well as carbon dioxide transport.
Alternative Names
vitamin B7; vitamin H; coenzyme R
UniProt
Immunogen
This antibody was raised by immunising mice with biotin conjugated to keyhole limpet haemocyanin.
Application Notes
This monoclonal antibody can be used to detect biotinylated proteins and antibodies, particularly if signal amplification is desired. For instance, PE-conjugated BK-1/39 antibody was used to target biotinylated anti-Ig beta antibody in a flow cytometric analysis (Levit-Zerdoun et al., 2016). This antibody was also successfully utilised in the chromatin immunoprecipitation (ChIP) of biotinylated sense or antisense strands of the saRNA duplex (Wu et al., 2016). BK-1/39 was used in in situ hybridisation as well; it detected biotinylated RNA probe complementary to the Epstein-Barr virus genome (de Lima et al., 2012).
Antibody First Published
Arnholdt et al. Receptor-mediated processing of epidermal growth factor in the trophoblast of the human placenta. Virchows Arch B Cell Pathol Incl Mol Pathol. 1991;61(2):75-80.PMID:1683723
Note on publication
This article describes the earliest found application of BK-1/39 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
