Cat. No.
MABL-525
Application
crystallization, Hemagglutination assay, Quantitative precipitin assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AC1001
From
Recombinant Antibody
Specificity
This antibody is specific for blood group antigen A.
Alternative Names
AC-1001
UniProt
Immunogen
The original antibody was produced by immunizing mice with A1 erythrocytes and with blood group A substance.
Application Notes
This antibody was used in a quantitative precipitin assay, a hemagglutination assay (HA), inhibition assays (by ELISA). In the HA with human erythrocytes, the antibody reacted with the A1 and A2 subtypes (showing double the sensitivity towards the A1 subtype) (Chen and Kabat, 1985; PMID: 2414276). The structure of the scFv version of this antibody was determined via crystallization (Thomas et al., 2002; PMID: 11679577).
Antibody First Published
Chen and Kabat. Immunochemical studies on blood groups. The combining site specificities of mouse monoclonal hybridoma anti-A and anti-B J Biol Chem. 1985 Oct 25;260(24):13208- 17 PMID:2414276
Note on publication
The original publication explores the specificities of mouse monoclonal hybridoma antibodies targeting blood group A and B antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
