Cat. No.
MABL-523
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
n/a
Clone No.
39-A11
From
Recombinant Antibody
Specificity
The antibody binds to hapten 4 with an affinity (Kd) of 10 ± 0.3 nM. The antibody has a Kcat of 0.67 s−1 and a Km of 1200 μM.
Alternative Names
UniProt
Immunogen
Application Notes
The antibody binds specifically to the bicyclo[2.2.2]octen moiety of hapten 4, and catalyses the pericyclic Diels- Alder reaction. The Diels-Alder is an organic chemical reaction between a conjugated diene and a dienophile which results in the formation of a substituted cyclohexene system. The Monoclonal antibody 39-A11 binds to the diene and dienophile in a reactive orientation, thereby catalysis the reaction by lowering the activation energy. The antibody has high affinity for hapten 4, and low affinity for the reaction end product, allowing it to be released once the reaction has terminated.
Antibody First Published
Romesberg FE, Spiller B, Schultz PG, Stevens RC. Immunological origins of binding and catalysis in a Diels-Alderase antibody. Science. 1998 Mar 20;279(5358):1929-33. PMID:9506942
Note on publication
Describes the generation and characterization of the three-dimensional structure of the monoclonal antibody 39-A11, a catalysis in the Diels-Alder reaction.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
