Cat. No.
MABL-521
Application
WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
S11B
From
Recombinant Antibody
Specificity
This antibody is specific for human beta-tubulin (no cross-reactivity with alpha-tubulin). Tubulin is the major constituent of microtubules. The alpha chain has a non-exchangeable GTP-binding site. Tubulin is the major constituent of microtubules. The beta-chain has an exchangeable GTP-binding site.
Alternative Names
TUBB; β-tubulin
UniProt
Q13509
Immunogen
The original antibody was generated via phage display from the Griffin.1 library by screening against Microtubule-Binding Proteins (MBPs) derived from HeLa S3 cells.
Application Notes
This antibody's original format (human scFv) was successfully used in a Western blot, and its anti-myc (9E10) complexed version was successfully used for in vitro immunofluorescence staining of β-tubulin in HeLa cells (Nizak et al., 2003; PMID: 14617357).
Antibody First Published
Nizak et al., Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo. Trafficking 2003; 4(11):739-753 PMID:14617357
Note on publication
The original publication demonstrates the use of recombinant antibodies selected through phage display against subcellular fractions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
