Cat. No.
MABL-520
Application
crystallography, SPR, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1/9
From
Recombinant Antibody
Specificity
This antibody is specific for the sequence TTLLRLPQKVFDAVVEAVARASLIPEFSDGFWTGSQLACWTNSETPWSYFPKISIYLRDENSSRSFRITILPQLYIQPMMGAGLNYEC of human Beta-secretase 2.
Alternative Names
DRAP; Asp 1; BACE2; AEPLC; ASP21; ALP56; ASP1; CDA13; UNQ418;PRO852; Memapsin-1; Aspartyl protease 1; Aspartic-like protease 56 kDa; Beta-site APP cleaving enzyme 2; Down region aspartic protease; Theta-secretase; Membrane-associated aspartic protease 1; Beta-site amyloid precursor protein cleaving enzyme 2
UniProt
Q9Y5Z0
Immunogen
The original antibody was raised by immunizing Swiss albino mice with human Beta- secretase 2 amino acids 244 to 333.
Application Notes
The structure of the original antibody was determined using x-ray crystallography. This antibody was used for surface plasmon resonance on Beta-secretase 2 (Banner et al., 2013; PMID:23695257). This antibody was used to stain BACE2 in immunofluorescence on pancreatic human islets (Rechsteiner et al., 2014; PMID:24905913).
Antibody First Published
Banner et al. Mapping the conformational space accessible to BACE2 using surface mutants and cocrystals with Fab fragments, Fynomers and Xaperones Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):1124-37. PMID:23695257
Note on publication
In the original paper BACE2-binding antibody Fab fragments, single-domain camelid antibody VHH fragments (Xaperones) and Fyn-kinase-derived SH3 domains (Fynomers) were used as crystallization helpers to obtain the first high-resolution structures of BACE2.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
