Cat. No.
MABL-514
Application
inhibit, ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia coli
Clone No.
cAbTEM13
From
Recombinant Antibody
Specificity
This antibody binds an enzyme called beta-lactamase produced by bacteria, which catalysis the breaking of the beta-lactam ring of beta-lactam antibiotics such as penicillin, cephalosporin, cephamycin and monobactam through hydrolysis. The breaking of the beta-lactam ring deactivates the antibacterial properties of the antibiotic molecule and provides antibiotic resistance in bacteria.
Alternative Names
β-Lactamase; bla; Beta-lactamase TEM; IRT-4; Penicillinase; TEM-1 β- lactamase; cAb-TEM13
UniProt
P62593
Immunogen
The original antibody was generated by immunizing adult dromedary (Camelus dromedarius) with TEM-1 β-lactamase.
Application Notes
The binding reactivity of this antibody to TEM-1 beta-lactamase was determined using ELISA. This antibody was capable of inhibiting beta-lactamase activity in vitro with an IC50 of 0.08 μM. The in vivo inhibition activity of this antibody to inhibit surface-exposed TEM-1 and prevent bacterial growth was assessed by preincubating cells expressing TEM-1 with increasing amounts of the antibody fragment. It was reported that when cells were preincubated with cAbTem13 before plating lost their ability to neutralize the antibiotic ampicillin (PMID: 11557473).
Antibody First Published
Conrath et al. β-Lactamase inhibitors derived from single-domain antibody fragments elicited in the Camelidae. Antimicrob Agents Chemother. 2001 Oct; 45(10): 2807–2812.PMID:11557473
Note on publication
Describes the generation of B-lactamase inhibitory antibodies derived from camels.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
