Cat. No.
MABL-515
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
5G8
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 436-450 of beta tubulin type III. IT binds to human, mice and rat beta tubulin type III. Its exact epitope is EAQGPK. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non- exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance.
Alternative Names
Tubulin beta-3 chain; beta tubulin type III; TUBB3; Tubulin beta-4 chain; Tubulin beta-III
UniProt
Q13509
Immunogen
The mouse version of this antibody was generated by immunizing mice with a synthetic peptide corresponding to the C-terminus of human beta III tubulin.
Application Notes
The mouse version of this antibody was used for a western blot on purified beta tubulin type III (Spano and Frankfurter, 2010; pmid:20466128). Neuronal cultures of leucine-rich repeat kinase 2 knock-out mice were immunostained using the mouse version of this antibody (Gillardon, 2009; pmid:19545277). To determine selective expression of beta-tubulin isotypes in taxane resistance, immunohistochemistry was preformed on human primary ovarian carcinoma tissues using the mouse version of this antibody (Ohishi et al, 2007; pmid:17343904). While determining the localization of βIII- tubulin in colorectal cancer specimens, the mouse version of this antibody was used for immunohistochemistry on colorectal cancer tissue (Portyanko et al, 2009; pmid:19360438). To investigate the protein antigens targeted by antibody diversity of infected mice with Trypanosoma cruzi, immunoblots were preformed against recombinant TcβTUB (Montalvão et al, 2018; pmid:29706955).
Antibody First Published
Ohishi et al. Expression of beta-tubulin isotypes in human primary ovarian carcinoma Gynecol Oncol. 2007 Jun;105(3):586-92. PMID:17343904
Note on publication
The purpose of this study was to evaluate the immunohistochemical expression of beta-tubulin isotypes using clinical samples of ovarian carcinoma treated by taxanes and to examine whether the protein levels of each of the beta-tubulin isotypes were correlated with the clinical features.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
