Cat. No.
MABL-511
Application
IP, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
AS30SS_Hu6
From
Recombinant Antibody
Specificity
This antibody recognizes human and mouse beta dystroglycan (β-DAG). The binding of this antibody to beta dystroglycan does not affect its binding to alpha dystroglycan. the Beta-dystroglycan is a transmembrane protein that plays an important role in connecting the extracellular matrix to the cytoskeleton. It plays an important role in linking dystrophin to the laminin-binding alpha-dystroglycan. It acts as a cell adhesion receptor in both muscle and non-muscle tissues. It also functions in cell adhesion- mediated signaling and implicated in cell polarity.
Alternative Names
Dystroglycan 1; Dag1; Beta-DG; β-DAG; BETA DAG; Dystroglycan; Dystrophin-associated glycoprotein 1; B-Dystroglycan; B Dystroglycan; Beta-dystroglycan; β dystroglycan; β-dystroglycan; AS30; humanized AS30
UniProt
Q14118; Q62165
Immunogen
The original antibody was generated by immunizing Balb/c and Trianni mice with human beta-dystroglycan. Humanized version was generated by grafting CDRs of parental mouse antibody AS30 onto human framework regions.
Application Notes
The binding specificity of this antibody for human and mouse beta dystroglycan (beta- DG) was confirmed using ELISA. This antibody is capable of immunoprecipitating both alpha-DG and beta- DG from C2C12 cell lysates, suggesting that the binding epitope of this antibody on beta dystroglycan is different from that of alpha dystroglycan. This antibody can also be used for the identification human beta- DG, mouse beta-DG and recombinant beta dystroglycan in a western blot. This antibody was also used for the immunofluorescent staining of unfixed human and mouse muscle tissues. This antibody was used in combination with anti-laminin-2 antibody C3_Hu10 to generate a duobody called TBTI (US11254737).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
