Cat. No.
MABL-510
Application
EMSA, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Chicken, Cow, Dog, Rat, Human, Mouse
Clone No.
15B8
From
Recombinant Antibody
Specificity
This antibody recognizes beta catenin. It does not react with plakoglobin. β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway.
Alternative Names
Catenin beta-1; Beta-catenin; β-catenin; β catenin
UniProt
P35222
Immunogen
This antibody was raised by immunizing mice with a recombinant full length β-catenin of chicken origin.
Application Notes
This antibody is recommended for the detection and analysis of beta-catenin via various methods, such as Western blot, flow cytometry and immunofluorescence of cells and tissues. For instance, the antibody 15B8 was utilized in labelling beta-catenin in oral mucosa samples via immunohistochemistry (Das et al., 2013). Another group used this antibody in the identification of beta- catenin in extracts from K562 cells via Western blots (Kotula et al., 2014). Furthermore, this antibody was applied during immunohistochemistry stainings of transverse sections taken through the midbrain region of 8–9ss embryos (Schiffmacher et al., 2016). Finally,beta-catenin is a very popular marker used to outline the cell membrane, this antibody might be utilized for such purposes (Zhang et al., 2017).
Antibody First Published
Smith et al. The effect of non-steroidal anti-inflammatory drugs on human colorectal cancer cells evidence of different mechanisms of action. Eur J Cancer. 2000 Mar;36(5):664-74. PMID:10738133
Note on publication
Tis article describes the use of the antibody 15B8 in the detection of beta-catenin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
