Cat. No.
MABL-504
Application
IP, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
13D6
From
Recombinant Antibody
Specificity
13D6 antibody recognizes human beta-2 adrenergic receptor. It was reported that this antibody recognizes extracellualr domains of the receptor (Han et al., 2012). Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds adrenaline with far grater affinity than noradrenaline.
Alternative Names
R11E1; Beta-2 adrenergic receptor; Beta-2 adrenoreceptor; Beta-2 adrenoceptor; β-2 adrenergic receptor; β-2 adrenoreceptor; β-2 adrenoceptor; β2AR; β2-AR; I3D6
UniProt
P07550
Immunogen
This antibody was raised by immunizing mice with beta-2 adrenergic receptor.
Application Notes
This antibody is recommended for the identification and analysis of the β2-adrenergic receptor. For instance, tt was used to immunostain vascular smooth muscle cells (VSMCs) against beta-2- adrenergic receptor to determine the fate of beta-2ARs ubiquitinated in a carvedilol responsive manner (Han et al., 2012). Furthermore, the antibody was utilized in the quantification of the surface level of β- 2ARs via ELISA (Han et al., 2012).
Antibody First Published
Han et al. MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound β(2)-adrenergic receptors. J Cell Biol. 2012 Nov 26;199(5):817-30. doi: 10.1083/jcb.201208192. Epub 2012 Nov 19. PMID:23166351
Note on publication
This article describes the use and characterisation of this anti-β2-adrenergic receptor antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
