Cat. No.
MABL-503
Application
FACS, in vivo, inhibition, IP, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
AIIB2
From
Recombinant Antibody
Specificity
The antibody specifically binds β1 integrin extracellular domain.
Alternative Names
integrin beta 1; Integrin beta-1-binding protein 1; Integrin cytoplasmic domain-associated protein 1 (ICAP-1)
UniProt
O14713
Immunogen
The original antibody was isolated by immunizing a Lewis rat with JAR choriocarcinoma cells.
Application Notes
The antibody immunoprecipitated the integrin subunits (Hall et al.,1990). This antibody was used for detection of β1 integrin expressed on CHO cells by flow cytometry (Takada et al., 1993; PMID: 7688727). The original format of the antibody or its F(ab′)2 fragments applied to single cells were capable of down-modulating β1 integrin signaling pathways (Wang et al., 1998; PMID: 9843973 and Weaver et al., 1997; PMID: 9105051). The antibody was used in immunofluorescence characterization of integrins in the HMT-3522 cells (Weaver et al., 1997; PMID: 9105051). The antibody inhibited tumor cell growth in several breast cancer cell lines (T4-2, MDA-MB-231, BT474, SKBR3, and MCF-7) and one nonmalignant cell line (S-1). Further, β1 integrin inhibition resulted in a significant loss of cancer cells, associated with a decrease in proliferation and increase in apoptosis, and a global change in the composition of residual colonies (Park et al., 2010; PMID: 16452209). The antibody was found to reduce the frequency of solid tumor stem cells. The antibody inhibited the growth of colon tumors and reduced the percentage of CD44+ cells. Tumors treated in mice showed a decrease when treated with the antibody.The humanized version of the antibody was constructed, showing potential for therapeutic uses (WO2009009114A3).
Antibody First Published
Hall et al. The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin. J Cell Biol (1990) 110 (6): 2175–2184.
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
