Cat. No.
MABL-502
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
22F12
From
Recombinant Antibody
Specificity
This antibody is specific for benzo[a]pyrene, which is an organic compound belonging to polycyclic aromatic hydrocarbon (PAH). Benzo[a]pyrene is one of the isomeric forms of benzopyrene. It comes from certain substances when they are not burned completely. It is found in car exhaust, smoke from wood fires, tobacco, oil and gas products, charred or grilled foods, and other sources. Traces of Benzopyrene are found in soil and water. It is also a causative agent for skin rashes, burning feeling, skin color changes, warts, bronchitis and even lung cancers.
Alternative Names
B[a]P; PAHs; PAH; Benzo[a]pyrene; 3 4-Benzopyrene; polycyclic aromatic hydrocarbons; chrysene; indeno[1 2 3-cd]pyrene; benzo[b]fluoranthene
UniProt
Immunogen
The original antibody was generated by using six B[a]P-protein conjugates B[a]P-6-C4-BSA, B[a]P-6-C4-KLH, B[a]P-7-C4-BSA, B[a]P-7-C4-KLH, B[a]P-10-C4-BSA and B[a]P-10-C4-KLH as immunogens for mouse immunizations.
Application Notes
This antibody was used in the development of a sensitive ELISA for the detection of benzopyrene in water. The average IC50 for B[a]P and corresponding detection limit at a signal noise (S/N) ratio of 3 was 65 ng/L and 24 ng/L. This antibody also cross reacts with chrysene,indeno[1,2,3-cd]pyrene, and benzo[b]fluoranthene (PMID: 15965533).
Antibody First Published
Matschulat et al. Development of a highly sensitive monoclonal antibody based ELISA for detection of benzo[a]pyrene in potable water. Analyst. 2005 Jul;130(7):1078-86.PMID:15965533
Note on publication
This paper describes the development of a sensitive indirect competitive ELISA for the detection of B[a]P in potable water.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
