Cat. No.
MABL-501
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
7
From
Recombinant Antibody
Specificity
The antibody is specific for BDE-47 and it does not cross react with BDE-153, BDE-154, BDE- 183, triclocarbon and TBBPA. BDE-47 is a polybrominated diphenyl ether which has been used as flame retardant.
Alternative Names
2 2′ 4 4′-tetra-bromodiphenyl ether; 4-Bromophenyl phenyl ether; CAS number： 101-55-3
UniProt
Immunogen
The original antibody was generated by immunizing an alpaca with a surrogate of BDE-47 covalently attached to a carrier protein. The resulting mRNA coding for the variable domain of heavy-chain antibodies were isolated, transcribed to cDNA, and cloned into a phagemid vector for phage display library construction. Selection of VHHs recognizing BDE-47 was achieved by panning under carefully modified conditions.
Application Notes
The specificity of the VHH fragment was confirmed by cELISA analysis. The VHH fragment showed increased thermal stability compared to polyclonal antibodies. The VHH antibody was immobilized onto an Au electrode and used for impedance biosensing, showing a detection limit for BDE-47 of approximately 0.79 μg/L (Bever et al., 2014; PMID： 25005746).
Antibody First Published
Bever et al. Development and utilization of camelid VHH antibodies from alpaca for 2,2',4,4'-tetrabrominated diphenyl ether detection Anal Chem. 2014 Aug 5;86(15)：7875-82. PMID：25005746
Note on publication
The paper describes the generation and characterization of the antibody, which is used for BDE-47 monitoring.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
