Cat. No.
MABL-487
Application
IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
6A7
From
Recombinant Antibody
Specificity
In the presence of nonionic detergent, the antibody avidly binds the monomeric form of Bax, but not Bax complexed with either Bcl-XL or Bcl-2. [6A7 ] Bax antibody only recognizes conformationally changed active Bax.
Alternative Names
Bcl-2-like protein 4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L- 4
UniProt
Q07812
Immunogen
KLH conjugated to peptides corresponding to amino acids 3–16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3–16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12–24 shared by Bax from both species (CGPTSSEQIMKTGA).
Application Notes
Antibody First Published
Hsu YT, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997 May 23;272(21)：13829-34. PMID：9153240
Note on publication
Describes the making of the species-independent Bax monoclonal antibodies, shows it recognised monomeric form of Bax, and analysed by immunoprecipitation followed by immunoblotting.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
