Cat. No.
MABL-477
Application
WB, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
S2
From
Recombinant Antibody
Specificity
This antibody binds a linear epitope comprising amino acids (VIQWLKEGVLGLVHEFK) of the human B7-H4.
Alternative Names
B7H4; B7 homolog 4; B7h.5; O8E; Immune costimulatory protein B7-H4; B7S1; Protein B7S1; B7x; T-cell costimulatory molecule B7x; VTCN1; V-set domain-containing T-cell activation inhibitor 1
UniProt
Q7Z7D3
Immunogen
The original antibody was generated by immunizing female white leghorn chickens with 50 µg of recombinant B7-H4 protein (Phe29 to Ala258) through intramuscular injection. The spleens of the chickens were harvested 7 days after the final immunization to construct an scFv antibody library. The antibody was isolated by panning against B7-H4 protein.
Application Notes
This antibody recognized intact wild type human B7-H4 and linear epitope comprising amino acids 'VIQWLKEGVLGLVHEFK' in an ELISA. This antibody was also capable or recognizing B7-H4 in a western blot assay. The binding of this antibody to B7-H4 protein can effectively neutralize the immunosuppression response induced by B7-H4 proteins, thus enabling T-cells to retain their activation status (PMID: 33716253).
Antibody First Published
Lin et al. Generation of avian-derived anti-B7-H4 antibodies exerts a blockade effect on the immunosuppressive response. Exp Anim. 2021 Aug 6;70(3):333-343. PMID:33716253
Note on publication
Describes the generation of chicken antibodies against human B7-H4.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
