Cat. No.
MABL-476
Application
functional assay, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
6H3
From
Recombinant Antibody
Specificity
This antibody is specific for the IgC region of human B7-H4.
Alternative Names
B7H4; B7 homolog 4; B7h.5; O8E; Immune costimulatory protein B7-H4; B7S1; Protein B7S1; B7x; T-cell costimulatory molecule B7x; VTCN1; V-set domain-containing T-cell activation inhibitor 1
UniProt
Q7Z7D3
Immunogen
The original antibody was raised by immunizing a DO11.10 mouse with B7-H4.
Application Notes
Humanized variants of this antibody 6H3V6 and 6H3V7 are available on request. This antibody is able to neutralize the effect of an B7-H4 blocking antibody (US9574000). Flow cytometry was performed on HEK293 cells that expressed full length human B7-H4 using this antibody (US9574000). This antibody induces robust internalization of B7-H4 expressed on the surface of both the B7-H4 transfected cell line and the B7-H4 positive breast cancer cell line SK-BR-3 (US9574000). Immunohistochemistry was performed on human tissue using this antibody (US9574000). An internalization assay was performed using this antibody on EG7 (B7-H4 negative cells), EG7.IG7 (B7-H4 low expressing cells), and EG7.IVB3 (B7-H4 high expressing cells) (US9574000). Antibody-Dependent Cell-Mediated Cytotoxicity assay was performed on EG7.hB7-H4 (clone IVB3) cells and SK-BR-3 cells using this antibody (US9574000). An ELISA was performed on B7-H4ECD his-tagged protein using this antibody (US9574000).
Antibody First Published
Langermann et al. Anti-human B7-H4 antibodies and their uses. US9574000
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
