Cat. No.
MABL-438
Application
Cryo-electron microscopy, inhibition
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
mAb1
From
Recombinant Antibody
Specificity
The antibody sterically blocks the arginase 1 active site near the exterior surface of the enzyme. Approximately 80% of all complexes identified were composed of two arginase 1 trimers and three antibodies, forming a 2 3 complex, while the remaining 20% of complexes were composed of two arginase 1 trimers and two antibodies; however, the epitope and paratope interactions between each antibody and arginase 1 monomers are identical. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.
Alternative Names
ARG1; Arginase-1; Liver-type arginase; Type I arginase
UniProt
P05089
Immunogen
The antibody is a human antibody identified via yeast display technologies and isolated after panning against arginase 1.
Application Notes
The structure and epitope mapping of the complex arginase antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of human arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki for the antibody against human Arg1 was 3.3 ± 0.3 nM (Palte et al., 2021; PMID: 34326456).
Antibody First Published
Palte et al. Cryo-EM structures of inhibitory antibodies complexed with arginase 1 provide insight into mechanism of action. Commun Biol. 2021 Jul 29;4(1):927. PMID:34326456
Note on publication
The paper describes the generation and characterization of the antibody. The antibody was used as probes for Human Arginase 1 conformational study.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
