Cat. No.
MABL-439
Application
Cryo-electron microscopy, inhibition
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
mAb5
From
Recombinant Antibody
Specificity
The antibody binds arginase 1. The binding of the antibody does not sterically block the Arginase 1 active site, but it interacts with an amino acid (Arg21) important to substrate binding by arginase 1, thereby inhibiting enzyme activity. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.
Alternative Names
ARG1; Arginase-1; Liver-type arginase; Type I arginase
UniProt
P05089
Immunogen
The antibody was generated by immunizing mice with recombinant arginase 1.
Application Notes
The structure and epitope mapping of the complex arginase antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of mouse arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki of the antibody against mouse Arg1 was 25 ± 1.7 nM (Palte et al., 2021; PMID: 34326456).
Antibody First Published
Palte et al. Cryo-EM structures of inhibitory antibodies complexed with arginase 1 provide insight into mechanism of action. Commun Biol. 2021 Jul 29;4(1):927. PMID:34326456
Note on publication
The paper describes the generation and characterization of the antibody. The antibody was used as probes for Human Arginase 1 conformational study.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
