Cat. No.
MABL-437
Application
in vivo, SPR, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CPR02
From
Recombinant Antibody
Specificity
The antibody is specific for Arg-2. Arg-2 is required for MUC2 post-transcriptional synthesis and secretion and, it may play a role in the production of mucus by intestinal cells.
Alternative Names
Human Anterior Gradient Protein 2 Homolog; Anterior gradient protein 2 homolog; AG-2; hAG-2; HPC8; Secreted cement gland protein XAG-2 homolog
UniProt
O95994
Immunogen
The antibody was isolated from the antibody library after three rounds of panning against residues 41-175 of recombinant human Agr2.
Application Notes
The scFv fragment of the antibody was able to bind the recombinant human Agr2 protein in ELISA analysis. The binding affinity of the scFv fragment to human Agr2 was measured by surface plasmon resonance (Kd= 4.6×10^−7 M). Immunofluorescence analysis of colon biopsy samples from a healthy donor was performed to verify the ability of CPR02 to recognize the human Agr2 antigen. Results showed the antibody revealed significant staining surrounding the crypts of the intestinal tissue. Further, the antibody was used for immunofluorescent staining of freshly frozen human biopsy samples of Ulcerative Colitis, the antibody produced a strong intensity signal in the colonic crypts region. The antibody was able to recognise the Agr2 antigen in vivo, as demonstrated by the results of the autoradiography of the complete intestinal tract of a mouse having colitis, injected with radiolabelled antibody (US20190309060A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
