Cat. No.
MABL-388
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
8E5D12A9
From
Recombinant Antibody
Specificity
The antibody is specific for alpha-SMA. α-SMA is an actin. Further, it is an intracellular protein that has both contractility and can aggregate into microfilaments, which are essential for the structure,motility and formation of skeletal cells. α-SMA, like all actins, is extremely conserved and is found in almost all mammals.
Alternative Names
CGMCC NO.22314; α-SMA; 1A4; Actin, aortic smooth muscle; Actin,aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTA2
UniProt
P62736
Immunogen
The original antibody was generated by immunizing mice with alpha-SMA.
Application Notes
The antibody specifically detected α-SMA protein by Western blot analysis. The antibody 8E5D12A9 and the commercial antibody 1A4 were used to stain leiomyomas and leiomyosarcoma.Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 8E5D12A9 was higher than the commercial antibody. The antibody and the commercially available antibody 1A4 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113845593A).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
