Cat. No.
MABL-389
Application
ICC, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D10
From
Recombinant Antibody
Specificity
This antibody binds human monomeric and high molecular weight forms of wild-type a-syn.Alpha-synuclein is a small 140 amino acid presynaptic neuronal protein, a major component of Lewy bodies and a member of the synuclein family. Misfolding, abnormal accumulation, and secretion of α-Synuclein (α-Syn) are closely associated with synucleinopathies, including Parkinson’s disease (PD). α-syn is abundant in the brain, and smaller amounts are found in the heart, muscles, and other tissues. In the brain, α-syn is found mainly at the tips of neurons in the specialized structures known as presynaptic terminals and has been shown to comprise up to ∼1% of total proteins in the neuronal cytosol. Recent evidence suggests that α-synuclein (α-syn) can contribute to the pathogenesis of amyotrophic lateral sclerosis (ALS).
Alternative Names
SNCA; NACP; PARK1; alpha-syn; α-Synuclein; α-syn; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor
UniProt
P37840
Immunogen
The original antibody was isolated from a human scFv phage display library by panning against human recombinant monomeric alpha-synuclein.
Application Notes
This antibody is capable of binding recombinant human alpha-synuclein in an ELISA.The intracellular binding of scFv D10 antibody to α-syn was studied by cotransfection of COS-7 cells with a D10-HA-NLS plasmid (hemagglutinin (HA) tag to detect scFv; nuclear localization signal (NLS) for retargeting to nucleus) and an α-syn plasmid. Immunocytochemical staining of HEK 293/α-syn/D10 cells reveals that the D10 intrabody has a predominantly cytoplasmic distribution and does not induce visible α-syn aggregate/inclusion formation. Transfection of D10 scFv gene into an HEK 293 cell line that overexpresses wild-type α-syn, showed that the D10 intrabody stabilizes detergent-soluble monomeric α-syn and inhibits the formation of detergent-insoluble high-molecular-weight α-syn species. It was seen that this antibody also ameliorated decreased cell adhesion characterized by α-syn-overexpressing cells (PMID:15564134). This antibody is capable of detecting total α-syn in a western blot (PMID: 26287258).
Antibody First Published
Zhou et al. A human single-chain Fv intrabody blocks aberrant cellular effects of overexpressed alpha-synuclein. Mol Ther. 2004 Dec;10(6):1023-31. PMID:15564134
Note on publication
Describes the generation of an intrabody against alpha-synuclein and evaluates the use of intrabodies as novel molecular therapeutics for synucleinopathies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
