Cat. No.
MABL-383
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Cow, Rabbit
Clone No.
Gal13
From
Recombinant Antibody
Specificity
This antibody is specific for α-gal epitopes (Galα1–3Galβ1–4GlcNAc-R) on glycosphingolipids.
Alternative Names
alpha gal; Galα1–3Galβ1–4GlcNAc-R; alpha gal; gal; α-gal
UniProt
Immunogen
This antibody was raised by immunising BALB/C mice, which express α-gal epitopes, with rabbit red cell membranes, on which α-gal is the major carbohydrate epitope.
Application Notes
This antibody binds to synthetic α-gal epitopes linked to BSA and to α-gal epitopes on glycolipid molecules from rabbit and bovine erythrocytes (Chen et al, 2000; Galili et al, 1987). However, in contrast to monoclonal anti-Gal antibodies originating from α1,3GT knockout mice [M86; B1H2; B4H2], this antibody does not bind to α-gal epitopes on carbohydrate chains of natural glycoproteins. This antibody has been used to confirm that Galαl-3Gal glycosphingolipids are expressed at low levels by human erythrocytes (Galili et al, 1987).
Antibody First Published
Galili et al. Identification of erythrocyte Gal alpha 1-3Gal glycosphingolipids with a mouse monoclonal antibody, Gal-13. J Biol Chem. 1987 Apr 5;262(10)：4683-8. PMID：2435715
Note on publication
Describes the original generation of this antibody, and its characterisation in ELISA analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
