Cat. No.
MABL-376
Application
ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
EnL5
From
Recombinant Antibody
Specificity
This antibody binds alpha-enolase, which is a glycolytic enzyme expressed in most tissues. It catalysis the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, it is involved in various processes such as growth control, hypoxia tolerance and allergic responses
Alternative Names
α-enolase; MBP-1; MPB-1; ENO1; NNE; ENO1L1; MBPB1; MPB1; Enolase 1; Non-neural enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Phosphopyruvate hydratase; Plasminogen-binding protein
UniProt
P06733
Immunogen
The original antibody was generated by immunizing female white leghorn (Gallus domesticus) chickens with 100 μg purified α-enolase in an equal volume of Freund's complete adjuvant by an intramuscular injection. The total RNA was extracted from the enlarged spleens for scFv antibody library construction. This clone was isolated by panning the scFv library against alpha-enolase.
Application Notes
The binding specificity of this antibody for alpha-enolase was done using ELISA. This antibody was also used for detection of α-enolase expressed in the PE089 cells using flow cytometry. This antibody was also used for immunofluorescent and immunocytochemical staining of α-enolase molecules expressed in PE089 cells (PMID:20655599).
Antibody First Published
Leu et al. Generation and characterization of anti-α-enolase single-chain antibodies in chicken. Vet Immunol Immunopathol. 2010 Oct 15; 137(3): 251–260. PMID:20655599
Note on publication
Describes the generation and characterization of single chain antibodies against alpha enolase.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
