Cat. No.
MABL-375
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Mycobacterium tuberculosis
Clone No.
B4m3
From
Recombinant Antibody
Specificity
This antibody is specific for the HspX antigen from Mycobacterium tuberculosis. This antigen acts as a chaperone, as it has a significant ability to suppress the thermal denaturation of alcohol dehydrogenase. Mycobacterium tuberculosis causes the disease tuberculosis.
Alternative Names
Acr; hspX; 14 kDa antigen; 16 kDa antigen; Antigen 16kDa (HspX); HSP 16.3; Nox16
UniProt
P9WMK1
Immunogen
This antibody was generated by first panning the Nkuku® library [26] against 20 μg/ml of the M. tuberculosis 16 kDa antigen. Second a sublibrary was created using error-prone PCR. Finally the mutant library was panned against 16 kDa M. tuberculosis antigen.
Application Notes
The binding of the scFv version of this antibody to the antigen was characterised by ELISA. Furthermore, surface plasmon resonance was used to determine the binding kinetics of this antibody on 16 kDa protein of M. tuberculosis (Sixholo et al, 2011; pmid:21349739).
Antibody First Published
Sixholo et al. Improving the characteristics of a mycobacterial 16 kDaspecific chicken scFv. Biologicals. 2011 Mar;39(2):110-6. PMID:21349739
Note on publication
A chicken single chain variable fragment (scFv) phage display library was panned against the mycobacterial 16 kDa antigen. Three fusion phages which bound specifically to the antigen were selected, each of which produced low signals in ELISA when secreted as a soluble scFv. One scFv was therefore chosen to be modified in an attempt to improve its binding.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
