Cat. No.
MABL-374
Application
crystallisation
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Nb25
From
Recombinant Antibody
Specificity
Binds to the transmembrane domain of the alpha-5 GABAA receptor. To see the exact epitope see the crystal structure (Miller et al, 2017). Nb25 straddles the neurotransmitter-binding site between adjacent subunits.
Alternative Names
GABRA5; Gamma-aminobutyric acid receptor subunit alpha-5; GABA(A) receptor subunit alpha-5
UniProt
P31644; P28472
Immunogen
A llama was immunized six times with recombinant 1D4-tagged human a1b3 GABAARpentamer. Nanobodies were selected by phage display, and the clones were then screened for binding to the receptor. From these clones Nb25 was selected.
Application Notes
The antibody was used to facilitate the crystallisation of the a5 GABAA receptor transmembrane domain where it was shown to form a 1080A2 area interface (approx.). Nb25 was used to increase the soluability and aid packing in the crystal. It has been shown that the binding of Nb25 does not affect the potentiation of the receptor. (Miller et al, 2017)
Antibody First Published
Miller et al Structural basis for GABAA receptor potentiation by neurosteroids Nat Struct Mol Biol. 2017 Nov;24(11):986-992 PMID:28991263
Note on publication
Reports crystal structures of a chimeric GABAA receptor in both the apo and pregnanolone-bound states. Discusses the generation of this antibody, and its use in crystallisation of the GABAA receptor.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
