Cat. No.
MABL-372
Application
Isotype
Engineer antibody
Species Reactivity
ELISA, FC
Clone No.
20000000000
From
Recombinant Antibody
Specificity
2E10 was generated by immunizing BALB/c mice with a DNA vaccine containing the humanalpha2 I-domain cDNAsequence within pVAC2. The alpha2 I-domain is expressed as a fusion protein withfragment C of tetanus toxoid (FrC-TT). The scFv was then created using a V-gene phage display library derived from RNA from mice splenocytes and isolated following selection on GST-I after 3 rounds of panning.
Alternative Names
CD49b; alpha2-beta1 integrin; glycoprotein IaIIa; GP IaIIa; VLA2; VLA-2;
UniProt
P17301
Immunogen
Human
Application Notes
scFv 2E10 can bind to the I (inserted) domain of recombinant alpha2 (GST- and His-), but cannot bind to the native epitope on platelets. This scFv also cross-reacts with and binds to the I-domain of alpha1 integrin. The alpha2 I-domain consists of ~200 amino acids and contains the ligand-binding site. The α2β1 integrin is expressed on platelets, fibroblasts, activated T lympho-cytes, endothelial cells and epithelial cells where its function is to act as a collagen and laminin receptor. 2E10 scFv can be used to detect alpha2 and alpha1 intergin I-domain in ELISA and FC studies.
Antibody First Published
Hughes et al. Platelet integrin alpha2 I-domain specific antibodies produced via domain specific DNA vaccination combined with variable gene phage display. Thromb Haemost. 2005Dec;94(6) 1318-26. PMID16411412
Note on publication
Describes the generation of mice scFvs by phage display and rabbit polyclonal antibodies raised against alpha2 intergin I-domain. The scFv specificity and reactivity was determined by ELISA and FC.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
