Cat. No.
MABL-368
Application
IP, WB, IF
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
1G5
From
Recombinant Antibody
Specificity
This antibody recognizes alpha catenin and binds to the domain between amino acids 135-226. α-catenin associates with the cytoplasmic domain of a variety of plasma membrane spanning cadherins that mediate cell-cell adhesion. The association of catenins to cadherins produces a complex that is linked to the actin filament network, which seems to be of primary importance for their cell-adhesion properties.
Alternative Names
Catenin alpha-1; Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13; α-catenin; α catenin; α(E)-catenin
UniProt
P35221
Immunogen
This antibody was raised by immunizing Balb/c mice with a 90 kDa MBP/alpha catenin fusion protein purified on an amylose column.
Application Notes
This antibody is recommended for the detection and analysis of alpha-catenin via various methods. For instance, it was used to identify alpha-catenin in MDA468 cells via immunofluorescence (Bajpai et al., 2009). It was also repeatedly demonstrated that 1G5 specifically detects alpha-catenin on Western blots (Johnson et al., 1993; Stadler et al., 2018).
Antibody First Published
Johnson et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res. 1993 Aug;207(2):252-60. PMID:8344378
Note on publication
This article describes the generation and characterization of the antibody 1G5.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
