Cat. No.
MABL-355
Application
crystallography, in vivo, inhibition, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
7B4
From
Recombinant Antibody
Specificity
The antibody is specific for ADAMTS5 and it recognises an epitope within the catalytic and disintegrin domains (amino acids 262-576). ADAMTS-5 is a protease involved in cartilage degradation in human OA patients.
Alternative Names
A disintegrin and metalloproteinase with thrombospondin motifs 5; Aggrecanase; ADAM-TS 5; ADAM-TS5; ADAMTS-5; A disintegrin and metalloproteinase with thrombospondin motifs 11; ADAM-TS 11; ADAMTS-11; ADMP-2; Aggrecanase-2; 7B4.1E11
UniProt
Q9UNA0
Immunogen
Application Notes
The antibody was characterized for binding affinity, species cross-reactivity and domain specificity. The antibody showed inhibitory potency (IC50) of 41.26 nM and humanization improved potency to 11.12 nM. The affinity of the original antibody and humanized version was measured, Kd= 0.280 nM and 0.205 nM respectively. Epitope mapping and selective binding to purified recombinant ADAMS5 were profiled by DELFIA. Selective binding of the antibody is demonstrated by immunocytochemistry using ADAMTS-5 and CHO cells. The antibody inhibited aggrecanolysis from human OA cartilage explants. The ability of the antibody to engage its target in vivo was assessed using labeled antibody in a preclinical OA model and whole body imaging was used to monitor biodistribution and cartilage penetration. The antibody treatment suppressed joint disease severity and mechanical allodynia. The crystal structure of the Fab fragment was determined (Larkin et al., 2015; PMID: 25800415).
Antibody First Published
Larkin et al. Translational development of an ADAMTS-5 antibody for osteoarthritis disease modification. Osteoarthritis Cartilage. 2015 Aug;23(8):1254-66. PMID:25800415
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
