Cat. No.
MABL-356
Application
IP, neutralizing, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Adeno-associated virus 2
Clone No.
A20
From
Recombinant Antibody
Specificity
The antibody recognizes a conformational epitope on assembled capsid proteins of AAV-2 but it fails to detect nonassembled or denatured capsid proteins.
Alternative Names
AAV-2 capsid proteins; Capsid protein VP1
UniProt
P03135
Immunogen
The original antibody was generated by immunizing BALB/c mice with a mixture of recombinant AAV capsid proteins (VP1, VP2, and VP3).
Application Notes
The clone was used to investigate the AAV assembly process on the cellular level. The clone was used to immunoprecipitate capsid proteins from extracts of [35S]methionine-labeled HeLa cells infected with AAV-2 and adenovirus type 2. Immunofluorescence was preformed on AAV-2/adenovirus type 2-coinfected HeLa cells using this antibody (Wistuba et al, 1997; pmid:8995658). Epitope sequences on the capsid surface were identified by enzyme-linked immunoabsorbent assay using AAV-2 mutants and AAV serotypes, peptide scan, and peptide competition experiments. The clone neutralizes infection following receptor attachment by binding an epitope formed during AAV-2 capsid assembly. The antibody bound empty and full AAV-2 particles in an ELISA. In a neutralization assay, incubation of rAAV-2-GFP with A20, on HeLa cells prevented transgene expression. The clone reacted also with AAV-3 as shown by ELISA (Wobus et al, 2000; pmid:10982375). The structure for the complex AAV-2 with Fab' fragment was determined by cryo-electron microscopy (McCraw et al, 2012; pmid:22682774).
Antibody First Published
Wistuba et al. Subcellular compartmentalization of adeno-associated virus type 2 assembly. J Virol. 1997 Feb;71(2):1341-52. PMID:8995658
Note on publication
The article describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
