Cat. No.
MABL-354
Application
inhibition
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
1-420 scFv
From
Recombinant Antibody
Specificity
Binds to the ADAMTS13 spacer domain. 1-420 binds to the same ADAMTS13 spacer domain loop segments that are required for binding of ADAMTS13 to VWF. ADAMTS13 is a zinc-containing metalloprotease which is secreted in blood where it is able to cleave VWF, a protein involved in blood clotting, to decrease the protein's activity.
Alternative Names
vWF-CP; ADAM-TS13; ADAMTS-13; C9orf8; VWFCP; ADAM metallopeptidase with thrombospondin type 1 motif 13
UniProt
A2ALB3
Immunogen
1-420 scFv was generated from a phage display library derived from human TTP patients expressing autoantibodies against ADAMTS13.
Application Notes
Mice transfected with the scFv show ADAMTS13 inhibition and accumulation of unprocessed UL-VWF (ultralarge VWF) multimers. 1-420 mediates inhibition by binding to the same spacer domain loop domain segments required for ADAMTS13 binding to VWF. Please note that this antibody is subject to a pending patent application by the The Trustees Of The University Of Pennsylvania (WO2016164468 A2).
Antibody First Published
Ostertag et al. ADAMTS13 autoantibodies cloned from patients with acquired thrombotic thrombocytopenic purpura: 2. Pathogenicity in an animal model Transfusion. 2016 Jul;56(7): 1775-85 PMID:27040023
Note on publication
"Describes the use of human anti-ADAMTS13 autoantibodies that function in mice to develop thrombotic thrombocytopenic purpura (TTP) disease model."
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
