Cat. No.
MABL-304
Application
colloidal gold immunochromatography, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
AbAb1-VD
From
Recombinant Antibody
Specificity
This antibody binds 25-hydroxyvitamin D. Vitamin D (calciferol) is an essential fat-soluble vitamin responsible for absorption and regulation of calcium and phosphorus, maintaining bone health, regulating immune function, preventing cardiovascular disease, and regulating fat and energy metabolism. It is important for the typical growth and development of bones and teeth and for improving resistance to certain diseases. Deficiency of vitamin may lead to osteoporosis or rickets.
Alternative Names
25-OH-VD; 25(OH)D; vitamin D; calciferol
UniProt
Immunogen
The original antibody was generated by subcutaneously immunizing New Zealand rabbits with a conjugate of 25-OH-VD and BSA emulsified with an equal amount of Freund's complete adjuvant.
Application Notes
This antibody binds 25-hydroxyvitamin D in an ELISA with an EC50 of 9.01 ng/mL and the IgG version of the antibody as a binding affinity in the sub-picomolar range. This antibody in combination with AbAb2-VD can be used in the development of double-antibody sandwich colloidal gold immunochromatography test strips, where this antibody is used to prepare Au-antibody complex and AbAb2-VD is used for T line coating.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
