Cat. No.
MABL-305
Application
colloidal gold immunochromatography, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
AbAb2-VD
From
Recombinant Antibody
Specificity
This antibody binds 25-hydroxyvitamin D. Vitamin D (calciferol) is an essential fat-soluble vitamin responsible for absorption and regulation of calcium and phosphorus, maintaining bone health, regulating immune function, preventing cardiovascular disease, and regulating fat and energy metabolism.
Alternative Names
25-OH-VD; 25(OH)D; vitamin D; calciferol
UniProt
Immunogen
The original antibody was generated by subcutaneously immunizing New Zealand rabbits with a conjugate of 25-OH-VD and BSA emulsified with an equal amount of Freund's complete adjuvant.
Application Notes
This antibody binds 25-hydroxyvitamin D in an ELISA with an EC50 of 4.72 ng/mL and the IgG version of the antibody as a binding affinity in the sub-picomolar range. This antibody in combination with AbAb1-VD can be used in the development of double-antibody sandwich colloidal gold immunochromatography test strips, where AbAb1-VD is used to prepare Au-antibody complex and AbAb2-VD is used for T line coating.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
