Cat. No.
MABL-297
Application
RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
mAb6kPGF
From
Recombinant Antibody
Specificity
The antibody binds to to 2,3-Dinor-6-keto-prostaglandin F1α with an IC50 of 2.2 pmol and an affinity (Kd) of 2.22 nM.
Alternative Names
2,3-dinor-6-keto-prostaglandin F1-alpha
UniProt
Immunogen
Human 2,3-dinor-6-keto PGF1α.
Application Notes
The antibody binds specifically to 2,3-Dinor-6-keto-prostaglandin F1α, a major urinary metabolite of PGI2 (prostacyclin). PG12 is a product of cyclooxygenase, and acts as a potent vasodilator and inhibitor of platelet aggregation. 2,3-Dinor-6-keto-prostaglandin F1α is a stable urinary metabolite of PGI2, and its levels in the urine can be measured using monoclonal antibodies in immunoassays, giving an indication of the levels of PGI2 produced in the body.
Antibody First Published
Hiramatsu M, Hayashi Y, Yamamoto S, Hayashi A, Sawada M, Hamanaka N, Yamashita K. Application of an alpha-side chain length-specific monoclonal antibody to immunoaffinity purification and enzyme immunoassay of 2,3-dinor-6-keto-prostaglandin F1α from human urine. Prostaglandins Leukot Essent Fatty Acids. 1994 Feb;50(2):69-79. PMID:8171070
Note on publication
Describes the preparation of a monoclonal antibody by immunising a mouse with 2,3-dinor-6-keto-prostaglandin F1α conjugated to bovine serum albumin, and its use for the detection of PG12 in human urine.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
