Cat. No.
MABL-296
Application
IP, RIA, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
n/a
Clone No.
57-2
From
Recombinant Antibody
Specificity
The antibody binds to 17ß estradiol with an affinity (Kd) of 2 nM.
Alternative Names
17-beta-estradiol; E2; ethynyl estradiol; oestradiol
UniProt
Immunogen
Human 17ß estradiol.
Application Notes
The antibody binds specifically to 17β-estradiol, the most potent member of the family of estrogens, which plays a role in both reproductive and non-reproductive physiological processes. 17β_x0002_estradiol is an important hormone during the pre-ovulatory phase of the menstrual cycle and in the prevention of osteoporosis in women. Monoclonal antibody directed against the hormone are used routinely in immunoassays to measure serum levels of 17β-estradiol.
Antibody First Published
Pajunen M, Saviranta P, Jauria P, Karp M, Pettersson K, Mäntsälä P, Lovgren T. Cloning, sequencing, expression and characterization of three anti-estradiol-17β Fab fragments. Biochim Biophys Acta. 1997 Mar20;1351(1-2):192-202. PMID:9116033
Note on publication
Describes the sequencing of three high affinity anti-estradiol-17β monoclonal antibodies and the determination of their crystal structures.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
