Cat. No.
MABL-295
Application
IP, RIA, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
n/a
Clone No.
17E12E5
From
Recombinant Antibody
Specificity
The antibody binds to 17ß estradiol with an ED50 of 76 pg/ml.
Alternative Names
17-beta-estradiol; E2; ethynyl estradiol; oestradiol
UniProt
Immunogen
estradiol-6-ethyl methoxy carbonyl (EMC)–bovine serum albumin (BSA).
Application Notes
The antibody binds specifically to 17β-estradiol, a mammalian estrogenic steroid hormone which is produced in the ovaries, placenta and testis. In women, the hormone plays an important role in the development of secondary sexual characteristics and maturation of long bones. The hormone is also responsible for the activation of certain oncogenes, and can be involved in the development of breast cancer and cancer of the uterine lining. Anti-estradiol antibodies can be useful in the detection of hormone concentrations in serum samples. This can be used therapeutically to monitor follicular maturation in cases of female infertility, and estradiol secreting tumours.
Antibody First Published
Bettsworth F, Monnet C, Watelet B, Battail-Poirot N, Gilquin B, Jolivet M, Menez A, Arnaud M, Ducancel F. Functional characterization of two anti-estradiol antibodies as deduced from modelling and site-directed mutagenesis experiments. J Mol Recognit. 2001 Mar-Apr;14(2):99-109. PMID:11301480
Note on publication
Describes the role of monoclonal antibodies in measuring concentrations of steroid hormones in human serum samples, and the characterization of two anti-estradiol monoclonal antibodies for this use.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
