Key features and details | |
Cat. No. | MABL-3142 |
Name | Anti-Tritrpticin mAbs |
Clone No. | AFD- 6C6D7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Pig |
Basic Information | |
Specificity | This antibody binds tritrpticin (VRRFPWWWPFLRR), which is a member of the cathelicidin family, a 13mer tryptophan-rich group of diverse cationic antimicrobial peptides found in neutrophil granules. It has a broad spectrum activity against a range of microorganisms, fungi, and protozoa. |
Alternative Name | C6; Prophenin and tritrpticin precursor |
UniProt | P51524 |
Immunogen | The original antibody was generated by immunizing female BALB/c mice of 6-8 weeks of age with 200 µg of K-tritrpticin-diphtheria toxoid conjugate emulsified with equal volume of complete Freund’s adjuvant. |
Application Notes | The binding characterization of this antibody was done using ELISA. In an indirect- binding ELISA where the peptides were directly immobilized on to the ELISA plate, mAb 6C6D7 could bind to tritrpticin and its N-terminal deleted antibacterial analogs at nanomolar concentrations. The binding affinity of this antibody to K-tritrpticin was analyzed using surface plasmon resonance and it was reported to be Kd= 350 nM. The binding of 6C6D7 to tritrpticin is characterized by negligible change in ΔGeq with respect to temperature that suggests that the antibody does not undergo any major conformational alteration during the binding of peptide antigen. This antibody was also used to bind tritripticin and related peptides to further analyze their anti-microbial activity (PMID: 24086578). |
Antibody First Published | Sharma et al. Putative bioactive motif of tritrpticin revealed by an antibody with biological receptor-like properties. PLoS One. 2013 Sep 24;8(9):e75582. PMID:24086578 |
Note on publication | Describes the generation of this antibody to study the mechanism of action of this antimicrobial peptide. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
