Anti-Triplex DNA mAbs

Cat. No.

MABL-3141

Name

Anti-Triplex DNA mAbs

Clone No.

AFD- Jel 466

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IF

Species Reactivity

Basic Information

Specificity

Jel 466 binds to triple-stranded DNA. The   preferred interacting partner is the triplex form of poly[d(TC)]. poly[d(GA)]   and there is also good binding to poly[d(TTC)].poly[d(GAA)]. Theantibody   binds weakly to the triplex derived from poly[d(G)].poly[d(C)] and no binding   is observed with poly[d(T)].poly[d(A)1.poly[d(T)]. Jel 466 does not bind to   poly[d(TC)].poly[d(Gm6A)], single-stranded DNAs, calf thymus DNA, or to   poly[d(TG)].poly[d(CA)] which are unable to form a triplex structure. Triplex   DNA has been known to form in vitro from synthetic pyrimidine-pruine tracts   and it is also thought to potentially have a biological function in cells.

Alternative Name

triple helix DNA; Jel-466; Jel466

UniProt

Immunogen

Jel 466 was prepared by immunizing mice with   poly[d(Tm5C)].poly[d(GA)].

Application Notes

Jel 466 can be used to detect triplex DNA in   cells by IF.

Antibody First   Published

Agazie et al. Characterization of a new   monoclonal antibody to triplex DNA and immunofluorescent staining of   mammalian chromosomes. J Biol Chem. 1994 Mar 4;269(9):7019-23. PMID:7509814

Note on publication

Describes the use of Jel 466 to study the distribution of triplex DNA in vivo and compares Jel 466 to another   anti-triplex DNA antibody, Jel 318.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.