Key features and details | |
Cat. No. | MABL-253 |
Name | Anti-MOG mAbs |
Clone No. | AFD-8.18-C5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, in vivo, WB, ELISA, FC, IF, IHC |
Species Reactivity | Cow, Guinea Pig, Monkey, Rat, Human, Mouse |
Basic Information | |
Specificity | The antibody binds specifically to MOG, recognising a conformational epitope. MOG is a transmembrane glycoprotein expressed on the surface of oligodendrocytes in the central nervous system, that plays an important role in maintaining the structure of the myelin sheath. Monoclonal antibodies that target MOG are involved in the pathogenesis of the demyelinating disorder multiple sclerosis (MS). Animals injected with anti-MOG antibodies have been shown to display symptoms of human MS, and can thereby be used as models to study the development of the disease. The epitope interacting with the antibody is expressed in rat, mouse, guinea pig, cow, monkey, and humans. |
Alternative Name | Myelin-oligodendrocyte glycoprotein; 8.18C5; 8-18C5 |
UniProt | Q63345 |
Immunogen | The original antibody was generated immunizing mice against Thy-1-depleted rat cerebellar glycoproteins. |
Application Notes | This antibody was detected in white matter tracts of the central nervous system by immununohistochemistry. The antibody detected MOG by western blot analysis (Linnington et al., 1984; PMID: 6207204). The specificity of the original format of the antibody and Fab fragment was confirmed by ELISA analysis (Litzenburger et al., 1998; PMID: 9653093 and Menge et al., 2011; PMID: 22093619). This antibody was used for detection of MOG expressed on B cells by flow cytometry (Na et al., 2021; PMID: 34149706). MOG from HeLa cells were immunoprecipitated with the antibody. |
Antibody First Published | Linnington et al. A novel myelin-associated glycoprotein defined by a mouse monoclonal antibody. J Neuroimmunol. Sep-Oct 1984;6(6):387-96. PMID:6207204 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
