Anti-Mad3 mAbs

Cat. No.

MABL-205

Name

Anti-Mad3 mAbs

Clone No.

AFD-N129A/6R

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, WB

Species Reactivity

Human, Mouse

Basic Information

Specificity

This antibody is specific for Mad3. Mad3 is an essential component of   the mitotic checkpoint. It is required for normal mitosis progression. The   mitotic checkpoint delays anaphase until all chromosomes are properly   attached to the mitotic spindle.

Alternative Name

Mitotic checkpoint serine/threonine-protein   kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic   checkpoint kinase MAD3L; Protein SSK1; N129A/6

UniProt

O60566

Immunogen

This antibody was raised against a   fusion protein of amino acids 116-206 of human Mad3.

Application Notes

This antibody is recommended for detection and analysis of Mad3 by   Immunocytochemistry and western blot. For instance, the mouse version of this   antibody was used to detect Mad3 by western blot in extracts of COS cells   transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3,   respectively) and untagged Kv2.1 plasmids.

Antibody First Published

Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

Describes the generation of a   library of recombinant monoclonal antibodies (R- mAbs) from a pool of   mAb-producing hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.